Where to Find Every Yellow Herb in Resident Evil 4's Separate Ways DLC

Playing through Resident Evil 4 Remake's Separate Ways DLC as Ada Wong, I quickly realized that survival hinges on more than just quick trigger fingers. The amber sample may be the overarching goal, but maintaining Ada’s health is the real mission moment-to-moment. Those precious yellow herbs, when combined with red and green herbs, permanently boost her maximum HP, and gathering all six triggers the Botanist challenge. It’s a hunt that took me across villages, castles, and waterways, and I’m here to retrace every step so you don’t miss a single one.

Chapter 1 throws you straight into the action with no yellow herbs in sight. I combed every corner of that opening area, but the plant life just isn’t there yet. The real herb hunt begins in Chapter 2, right after I took down the first Bullhead Brute. My heart was still pounding as I pushed through the gate that the brute had smashed open. On the left side, nestled atop a stack of wooden pallets, I found my first yellow herb. It was almost camouflaged against the worn timber—an easy miss if you’re sprinting past. Later in the same chapter, as I made my way back to the church, I remembered the burial plot inside the church gates. Tucked at the left end of that somber little graveyard, a second yellow herb waited. The quiet atmosphere of the church gave this discovery a strangely serene moment amid the chaos.

Chapter 3 took me into a darker, more claustrophobic section: the hallway leading to the room where Leon and Luis were chained up in the main story. Just before the ramp that descends into that prison-like space, I noticed a shadowy corner on the right. Stacked on some boxes, almost invisible unless you deliberately scan the area, sat a yellow herb. The tension of nearing that infamous room made the find feel like a tiny victory. I grabbed it and moved on, grateful for the extra heath to come.

Chapter 4 changed the scenery dramatically with those towering castle walls. After exiting the courtyard and watching Ada grapple across the wooden bridge that El Gigante famously destroyed in the main game, I arrived at the last section of the wall. Hugging the right-hand wall, I saw it: a yellow herb growing stubbornly against the stone. The contrast of vivid green and yellow against gray stone made it pop, but adrenaline from the bridge crossing can easily blind you. I paused, took a deep breath, and picked it up.

Chapter 5 ushered in the damp, echoing corridors of Gregorio’s waterway. Early on, following Luis’s notes, I encountered a group of zealots guarding a walkway. After a frantic firefight near some breakable crates, I noticed something nestled to the right of those crates—a yellow herb, shining faintly in the torchlight. The placement felt deliberate, a reward for clearing a tough ambush. I pocketed it quickly and pressed on, the waterway's ambient drips adding to the sense of urgency.

Chapter 6 begins the countdown to the wrecking ball fight and the factory descent. Almost immediately after opening the double doors to the upper platform, I spotted a yellow herb lying on the ground to the left. No enemies were around, no crates to smash—just the herb, right there, as if the developers were throwing me a bone before the upcoming chaos. I appreciated the straightforward find.

And that’s the full set. Chapter 7 carries none of these golden plants, which makes sense given the frantic climax. The full breakdown goes like this: Chapter 2 gifts you two herbs, while Chapters 3, 4, 5, and 6 each hold one. Mixing any of these with a red and green herb not only fully restores Ada’s health but also nudges that permanent HP bar upward. After consuming the sixth combined herb, the Botanist challenge pops and Ada’s vitality maxes out—an absolute game-changer for tougher difficulties.

A quick summary table makes this easier to reference:

My biggest takeaway from this scavenger hunt? Slow down. The Separate Ways campaign moves briskly, and these herbs are almost always tucked just off the main path, demanding a moment’s pause. The developers clearly want you to explore every shadowy corner, and if you’re like me, you’ll find the rhythm of combat and discovery deeply satisfying. Whether you’re aiming for the Botanist challenge or just want every advantage against Saddler’s forces, these yellow herbs are worth the short detours. Ada’s resilience becomes your lifeline, and with all six in your inventory, you’ll feel nearly unstoppable. Happy hunting.